Una pipeta es una herramienta básica de laboratorio concebida para la manipulación precisa de líquidos. Su función principal consiste en medir y transferir con exactitud volúmenes predeterminados de líquidos, tarea indispensable en múltiples ensayos y análisis científicos. Existen diversos tipos de pipetas adaptados a aplicaciones específicas.
Pipeta electrónica: Una pipeta electrónica es un instrumento de precisión que mide y transfiere por medios electrónicos pequeños volúmenes de líquido. Proporciona mayor control, automatización, pantallas digitales, modos programables y diseños ergonómicos para un pipeteo uniforme en variadas aplicaciones científicas.
Pipeta mecánica: Una pipeta mecánica, o manual por desplazamiento de aire, constituye una herramienta fundamental para mediciones volumétricas exactas. Accionada manualmente, depende de un émbolo para generar vacío y aspirar o dispensar líquidos. Muy utilizada en biología, química y medicina, es una solución económica para preparación de muestras y valoraciones, donde la exactitud depende de la destreza del operador.
Características imprescindibles de una pipeta:
- Diseño ergonómico para un agarre confortable
- Mecanismo de ajuste volumétrico calibrado para garantizar exactitud
- Controles sencillos para un manejo eficiente
- Esterilizable en autoclave y resistente a sustancias químicas
MCEP
Multi-channel Electronic Pipette
Specifications:
- Lightweight and ergonomic design allows effortless single-handed pipetting
- Designed for handling high throughput and multi-samples assays
- Ideal for processing large-scale samples
- Leak-tight design and compatible with any brand tips
- Autoclavable liquid suction nozzles
- Has a powerful memory function that allows saved set programs
- Available in 8 or 12 channels
A200
A-Series Mechanical Pipette
Specifications:
- 4 digits with magnifying shape
- Chemical-resistant
- 121°C sterilization
- Very low operating force
- Colour indications for pipette volume
- Positioned for perfect ergonomics
- Only few turns to reach your desired volume
SCEP
Single-channel Electronic Pipette
Specifications:
- Innovative electronic control system
- High-performance motor for a precise one-step volume adjustment (0.5μl~5ml)
- Strict quality control to minimize error and ensure the consistency between vertical and horizontal pipetting
- Features a high-definition visual screen that displays pipette volume and workflow
- Highly compatible with various tips common in the laboratory
A pipette is a laboratory tool designed for precise measurement and transfer of liquid volumes. Its primary function is to facilitate accurate dispensing of liquids in various scientific experiments.
Pipettes come in various types, such as air-displacement and positive-displacement pipettes. They differ in their mechanisms, making them suitable for different applications. Air-displacement pipettes are commonly used for general liquid handling, while positive-displacement pipettes are ideal for viscous or volatile liquids.
Consider factors like the liquid's viscosity, required precision, and the pipette's volume range. Choose a pipette type that aligns with the characteristics of your experiment
Pipettes operate based on displacement principles. Air-displacement pipettes displace liquid with air, while positive-displacement pipettes use a disposable tip to directly displace the liquid. Calibration ensures accuracy in volume delivery.
Common errors include incorrect pipetting technique, temperature variations, and improper calibration. Minimize errors by using proper technique, maintaining consistent environmental conditions, and regularly calibrating pipettes.
Pipettes should be calibrated at regular intervals, typically every 3-12 months, depending on usage. Calibration involves comparing the pipette's performance against a standard using precision test equipment.
Use proper pipetting techniques, such as maintaining a vertical position and releasing the plunger slowly. Pre-wet tips to enhance accuracy, and avoid over-pipetting or under-pipetting
Environmental conditions can affect the density of liquids and the functioning of air-displacement pipettes. It is crucial to work within specified temperature and humidity ranges to maintain accuracy.
Clean pipettes regularly with mild detergent and distilled water. For decontamination between different liquids, use appropriate disinfectants or methods recommended by the laboratory.
Some pipettes are designed to handle corrosive or volatile substances. Check the pipette specifications and ensure compatibility. Using specialized pipettes for such substances is recommended.
Troubleshoot issues by checking for proper calibration, inspecting seals and O-rings, ensuring proper tip attachment, and verifying user technique. Refer to the pipette manual or seek assistance if problems persist.