I. Introduction
A Polymerase Chain Reaction (PCR) cabinet provides DNA and RNA contaminant-free environment through HEPA filters and UV decontamination system. It is a vital part of PCR laboratories that require applications such as PCR mastermix preparation and UV decontamination of PCR consumables. Having enough equipment knowledge is crucial in making the next step for your PCR laboratory.
II. PCR Cabinet Guide to Models
| PCR Cabinet |
|
|
|---|---|---|
| Application | Provide a sterile environment during PCR mastermix preparation | |
| Size Available | 2 ft. | 3 ft. and 4 ft. |
| Control System |
Rocker Switches with UV timer 
|
Sentinel™ Silver Microprocessor 
|
| Blower | AC centrifugal motor fan | |
| Filters | Main filter: HEPA / H13 main filter Pre-filter: Washable polyurethane fibers with 85% arrestance |
|
| Construction | Main body: Electro-galvanized steel with oven-baked epoxy-polyester powder and antimicrobial coating Work zone: Stainless steel grade 304 |
|
| Airflow Velocity | 0.30 m/s (60 fpm) | |
| Accessories |
|
|
III. Must-have Features of a PCR Cabinet
HEPA-filtered laminar flow
Protection against cross-contamination within the main chamber
UV decontamination technology
UV interlock system
Low noise level
Antimicrobial coating
IV. Factors to Consider When Buying a PCR Cabinet
The main purpose of a PCR cabinet is to provide a HEPA-filtered vertical laminar flow to purge the work area of contaminants between amplification and during preparatory procedures.
Factors to consider when purchasing a PCR Cabinet
Durability
Performance
Ergonomics
Filter efficiency
Effective UV decontamination technology
Energy-efficient
Low maintenance cost
After-sales service
Value for money
Longer warranty period
Typical questions to ask before buying
For what application/s would the PCR cabinet be used?
What is the preferred work surface material and construction?
What is the type of support stand needed?
How much is the installation cost and how difficult is it to install?
How much is the operational cost?
What is the required cabinet size? Will it fit in the lab?
Will the PCR cabinet fit on hallways, doors, elevators, and other paths?
Reasons to purchase a new one
Setting up a new laboratory facility
Replacing an old and less efficient unit that has a high operational cost
Defective unit due to electronic or mechanical malfunctions
PCR cabinet repeatedly failed the certification which means it’s no longer safe to use
Additional unit due to an increase in laboratory works
V. Safety Tips When Using a PCR Cabinet
Do not use PCR cabinets to process biohazard samples. PCR cabinets only protect samples inside the work zone from external airborne contamination. They do not protect the operator.
Allow the purge cycles. Leave the blower on for at least 3 minutes before and after use to purge the work zone of any contaminants.
Only arms and hands are allowed inside the work zone. Operators must always bear in mind that head and shoulders should not be inside the work zone and doing so might potentially contaminate the samples.
Do not spray anything on the back wall. This can damage the HEPA/ULPA filter and cause leakage.
Do not place your body parts directly above sensitive samples. This may be a possible cause of product contamination.
Do not store your stuff inside the cabinet. Overloading the cabinet with unnecessary items can affect the cabinet’s airflow and containment.
Only sterile materials should be placed inside the clean work area. Make sure that all your items are disinfected properly to avoid possible contamination of the work bench.
Identify the proper location for the cabinet. External airflow disturbances (doors, excessive human traffic, windows, diffusers, air conditioner outlets) can compromise containment. Minimize disturbances to the airflow barrier.
Do not use the Bunsen burner inside the cabinet. The resulting buoyancy effect will affect the cabinet’s airflow and containment. When necessary, low pilot light type electric burners may be used.
Wear Personal Protective Equipment (PPE) properly. Wearing a back-fastened lab coat (to protect the operator from splashes) as well as double gloving (over the cuffs) should be practiced.